Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment
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Published:2022-09-26
Issue:21
Volume:106
Page:7173-7185
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ISSN:0175-7598
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Container-title:Applied Microbiology and Biotechnology
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language:en
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Short-container-title:Appl Microbiol Biotechnol
Author:
Rizzo CarmenORCID, Perrin ElenaORCID, Poli AnnaritaORCID, Finore IlariaORCID, Fani RenatoORCID, Lo Giudice AngelinaORCID
Abstract
Abstract
A synergistic approach using cultivation methods, chemical, and bioinformatic analyses was applied to explore the potential of Pseudoalteromonas sp. S8-8 in the production of extracellular polymeric substances (EPSs) and the possible physiological traits related to heavy metal and/or antibiotic resistance. The effects of different parameters (carbon source, carbon source concentration, temperature, pH and NaCl supplement) were tested to ensure the optimization of growth conditions for EPS production by the strain S8-8. The highest yield of EPS was obtained during growth in culture medium supplemented with glucose (final concentration 2%) and NaCl (final concentration 3%), at 15 °C and pH 7. The EPS was mainly composed of carbohydrates (35%), followed by proteins and uronic acids (2.5 and 2.77%, respectively) and showed a monosaccharidic composition of glucose: mannose: galactosamine: galactose in the relative molar proportions of 1:0.7:0.5:0.4, as showed by the HPAE-PAD analysis. The detection of specific molecular groups (sulfates and uronic acid content) supported the interesting properties of EPSs, i.e. the emulsifying and cryoprotective action, heavy metal chelation, with interesting implication in bioremediation and biomedical fields. The analysis of the genome allowed to identify a cluster of genes involved in cellulose biosynthesis, and two additional gene clusters putatively involved in EPS biosynthesis.
Key points
• A cold-adapted Pseudoalteromonas strain was investigated for EPS production.
• The EPS showed emulsifying, cryoprotective, and heavy metal chelation functions.
• Three gene clusters putatively involved in EPS biosynthesis were evidenced by genomic insights.
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Reference48 articles.
1. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M, Edalatmand A, Huynh W, Nguyen A-LV, Cheng AA, Liu S, Min SY, Miroshnichenko A, Tran H-K, Werfalli RE, Nasir JA, Oloni M, Speicher DJ, Florescu A, Singh B, Faltyn M, Hernandez-Koutoucheva A, Sharma AN, Bordeleau E, Pawlowski AC, Zubyk HL, Dooley D, Griffiths E, Maguire F, Winsor GL, Beiko RG, Brinkman FSL, Hsiao WWL, Domselaar GV, McArthur AG (2020) CARD 2020: antibiotic resistome surveillance with the Comprehensive Antibiotic Resistance Database. Nucleic Acids Res 48:D517–D525 2. Berendonk TU, Manaia CM, Merlin C, Fatta-Kassinos D, Cytryn E, Walsh F, Bürgmann H, Sørum H, Norström M, Pons M-N (2015) Tackling antibiotic resistance: the environmental framework. Nat Rev Microbiol 13:310–317 3. Blumenkrantz N, Asboe-Hansen G (1973) New methods for quantitative determination of uronic acids. Anal Biochem 54:484–489 4. Bosi E, Fondi M, Maida I, Perrin E, de Pascale D, Tutino ML, Parrilli E, Lo Giudice A, Filloux A, Fani R (2015) Genome-scale phylogenetic and DNA composition analyses of Antarctic Pseudoalteromonas bacteria reveal inconsistencies in current taxonomic affiliation. Hydrobiologia 761:85–95 5. Bosi E, Fondi M, Orlandini V, Perrin E, Maida I, de Pascale D, Tutino ML, Parrilli E, Lo Giudice A, Filloux A, Fani R (2017) The pangenome of (Antarctic) Pseudoalteromonas bacteria: evolutionary and functional insights. BMC Genomics 18(1):93
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