Detection of viral antibodies in camel sera using magnetic particle spectroscopy

Author:

Friedrich Bernhard,Vogel Patrick,Rückert Martin A.,Lyer Stefan,Günther Johanna,Wernery Ulrich,Joseph Sunitha,Müller Judith,Behr Volker C.,Alexiou Christoph,Tietze RainerORCID

Abstract

Abstract Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections. Key points • COMPASS (critical offset magnetic particle spectroscopy) allows the fast detection of antibodies. • Magnetic nanoparticles can be adapted by exchange of the linked bait molecule. • Antibodies could be detected in camel sera without washing steps within seconds.

Funder

Manfred-Roth-Stiftung, Fürth

Forschungsstiftung Medizin am Universitätsklinikum Erlangen

Hans Wormser, Herzogenaurach

Deutsche Forschungsgemeinschaft

Universitätsklinikum Erlangen

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference33 articles.

1. Al-Numair N, Theyab A, Alzahrani F, Shams A, Al-Anazi I, Oyouni A, Al-Amer O, Mavromatis H, Saadeldin I, Abdali W, Hawsawi Y (2022) Camels’ biological fluids contained nanobodies: promising avenue in cancer therapy. Cancer Cell Int 22 https://doi.org/10.1186/s12935-022-02696-7

2. Baumann S, Ceglarek U, Fiedler GM, Lembcke J, Leichtle A, Thiery J (2005) Standardized approach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chem 51(6):973–980. https://doi.org/10.1373/clinchem.2004.047308

3. Biederer S, Knopp T, Sattel TF, Ludtke-Buzug K, Gleich B, Weizenecker J, Borgert J, Buzug TM (2009) Magnetization response spectroscopy of superparamagnetic nanoparticles for magnetic particle imaging. J Phys D Appl Phys 42(20) https://doi.org/10.1088/0022-3727/42/20/205007

4. Bommana S, Jelocnik M, Borel N, Marsh I, Carver S, Polkinghorne A (2019) The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock. J Med Microbiol 68(4):627–632. https://doi.org/10.1099/jmm.0.000951

5. Cáceres-Martell Y, Fernández-Soto D, Campos-Silva C, García-Cuesta EM, Casasnovas JM, Navas-Herrera D, Beneítez-Martínez A, Martínez-Fleta P, Alfranca A, Sánchez-Madrid F, López GE, Vilches C, Jara-Acevedo R, Reyburn HT, Frade JMR, Valés-Gómez M (2021) Bead-assisted SARS-CoV-2 multi-antigen serological test allows effective identification of patients. medRxiv:2021.04.08.21254348 https://doi.org/10.1101/2021.04.08.21254348

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