Abstract
Abstract
Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φn on selected critical point of the fifth higher harmonic (n = 5th). Here, positive sera display highly significant signal increase over the control or negative sera. Furthermore, a clear distinction could be made in antibody detection as an immune response to closely related viruses (SARS-CoV2 and MERS). Using modified MNPs along with COMPASS offers a fast and reliable method that is less cost intensive than current technologies and offers the possibility to be quickly adapted in case of new occurring viral infections.
Key points
• COMPASS (critical offset magnetic particle spectroscopy) allows the fast detection of antibodies.
• Magnetic nanoparticles can be adapted by exchange of the linked bait molecule.
• Antibodies could be detected in camel sera without washing steps within seconds.
Funder
Manfred-Roth-Stiftung, Fürth
Forschungsstiftung Medizin am Universitätsklinikum Erlangen
Hans Wormser, Herzogenaurach
Deutsche Forschungsgemeinschaft
Universitätsklinikum Erlangen
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Cited by
1 articles.
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