Secretome-based screening of fusion partners and their application in recombinant protein secretion in Saccharomyces cerevisiae
Author:
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s00253-021-11750-9.pdf
Reference29 articles.
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2. Bae JH, Sung BH, Kim HJ, Park SH, Lim KM, Kim MJ, Lee CR, Sohn JH (2015) An efficient genome-wide fusion partner screening system for secretion of recombinant proteins in yeast. Sci Rep 5:12229. https://doi.org/10.1038/srep12229
3. Bae JH, Sung BH, Seo JW, Kim CH, Sohn JH (2016) A novel fusion partner for enhanced secretion of recombinant proteins in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 100(24):10453–10461. https://doi.org/10.1007/s00253-016-7722-2
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
5. Choi ES, Sohn JH, Rhee SK (1994) Optimization of the expression system using galactose-inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 42(4):587–594
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