Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis

Author:

Maurício da Fonseca Maria João,Jurak Edita,Kataja Kim,Master Emma R.,Berrin Jean-Guy,Stals Ingeborg,Desmet Tom,Van Landschoot Anita,Briers YvesORCID

Funder

Research fund of the University College Ghent and Ghent University

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference47 articles.

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2. Alvarez TM, Goldbeck R, dos Santos CR, Paixão DAA, Gonçalves TA, Franco Cairo JPL, Almeida RF, de Oliveira Pereira I, Jackson G, Cota J, Büchli F, Citadini AP, Ruller R, Polo CC, de Oliveira Neto M, Murakami MT, Squina FM (2013) Development and biotechnological application of a novel endoxylanase family GH10 identified from sugarcane soil metagenome. PLoS One 8:e70014. https://doi.org/10.1371/journal.pone.0070014

3. Borsenberger V, Dornez E, Desrousseaux M-L, Massou S, Tenkanen M, Courtin CM, Dumon C, O’Donohue MJ, Fauré R (2014) A 1H NMR study of the specificity of α-l-arabinofuranosidases on natural and unnatural substrates. Biochim Biophys Acta 1840:3106–3114. https://doi.org/10.1016/j.bbagen.2014.07.001

4. Broberg A, Duus J, Thomsen KK, Ferre H (2000) A novel type of arabinoxylan arabinofuranohydrolase isolated from germinated barley. Analysis of substrate preference and specificity by nano-probe NMR. Eur J Biochem 6641:6633–6664. https://doi.org/10.1046/j.1432-1327.2000.01758.x

5. Cairo JPLF, Leonardo FC, Alvarez TM, Ribeiro DA, Büchli F, Costa-Leonardo AM, Carazzolle MF, Costa FF, Leme AFP, Pereira GAG, Squina FM (2011) Functional characterization and target discovery of glycoside hydrolases from the digestome of the lower termite Coptotermes gestroi. Biotechnol Biofuels 4:50. https://doi.org/10.1186/1754-6834-4-50

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3. High-Throughput Generation of Product Profiles for Arabinoxylan-Active Enzymes from Metagenomes;Applied and Environmental Microbiology;2020-11-10

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