Glass bead system to study mycotoxin production of Aspergillus spp. on corn and rice starches

Author:

Inotai Katalin,Bata-Vidács IldikóORCID,Tóth Ákos,Kosztik Judit,Varga Mónika,Szekeres András,Nagy István,Nagy István,Dobolyi Csaba,Mörtl Mária,Székács András,Kukolya József

Abstract

Abstract Mycotoxin production by aflatoxin B1 (AFB1) -producing Aspergillus flavus Zt41 and sterigmatocystin (ST) -hyperproducer Aspergillus creber 2663 mold strains on corn and rice starch, both of high purity and nearly identical amylose-amylopectin composition, as the only source of carbon, was studied. Scanning electron microscopy revealed average starch particle sizes of 4.54 ± 0.635 µm and 10.9 ± 2.78 µm, corresponding to surface area to volume ratios of 127 1/µm for rice starch and 0.49 1/µm for corn starch. Thus, a 2.5-fold difference in particle size correlated to a larger, 259-fold difference in surface area. To allow starch, a water-absorbing powder, to be used as a sole food source for Aspergillus strains, a special glass bead system was applied. AFB1 production of A. flavus Zt41 was determined to be 437.6 ± 128.4 ng/g and 90.0 ± 44.8 ng/g on rice and corn starch, respectively, while corresponding ST production levels by A. creber 2663 were 72.8 ± 10.0 µg/g and 26.8 ± 11.6 µg/g, indicating 3–fivefold higher mycotoxin levels on rice starch than on corn starch as sole carbon and energy sources. Key points A glass bead system ensuring the flow of air when studying powders was developed. AFB1 and ST production of A. flavus and A. creber on rice and corn starches were studied. 3–fivefold higher mycotoxin levels on rice starch than on corn starch were detected.

Funder

National Research, Development and Innovation Office

Eötvös Loránd Research Network

Innovációs és Technológiai Minisztérium

Eszterhazy Karoly Catholic University

Publisher

Springer Science and Business Media LLC

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