Cultivation of early life history stages of Porphyra dioica from the British Isles

Abstract

AbstractBladed Bangiales of the genus Porphyra/Pyropia are highly valuable red algae and extensively farmed in South East Asia. Interest is rising in cultivating species local to the North East Atlantic but the control of the heteromorphic life cycle of native species remains difficult as previous studies reported high inter- and intraspecific variability in required cultivation conditions. Here, working with Porphyra dioica from a UK source population, we conducted a series of experiments investigating the influence of substrate, temperature, photoperiod and light intensity on the development of early life history stages (conchocelis (filamentous sporophyte) and young thalli (gametophyte)). Special focus was the influence of temperature and photoperiod on mature conchocelis to induce a conchospore mass release—the current bottleneck of European Porphyra cultivation. Sporophytes grew largest on an oyster shell substrate and under long day conditions at 18 °C. A decrease in temperature from 18 to 9 °C initiated a mass conchospore release (498 ± 146 spores mL−1) from a P. dioica conchocelis culture grown in suspension. Released conchospores germinated into small thalli on nylon ropes, with best growth (7.2 ± 0.9% day−1) at low temperatures of 9 °C. Conchospore germination increased with decreasing light intensity but germination success was generally very low (< 5%), indicating the cultivation protocol needs further improvement. Our results reflect the adaptation of P. diocia to seasonal environmental conditions in temperate regions and the importance of these conditions for the successful cultivation. We are the first to describe a mass conchospore release for P. diocia growing in suspension which has important implications for commercial production.