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3. Because they are not direct measurements of absorbance of circularly polarized light, these methods are sometimes called indirect methods [4]. We consider this to be a misnomer because circular dichroism can be detected in a single ellipticity measurement while detection using absorbance of first LCP and then RCP (the so-called direct method which we refer to here as the absorbance method) always requires a combination of two observations. Instead of using the term indirect we prefer the term ellipticity methods.
4. J. Badoz, in F. Ciardelli and P. Salvadori (Eds.), Fundamental Aspects and Recent Developments in Optical Rotatory Dispersion and Circular Dichroism, Heyden and Son Ltd., London, 1973.
5. The elliptically polarized beam can be analyzed in terms of a linearly polarized component plus an additional circularly polarized component. The quarter wave plate converts the circular component into a linearly polarized component oriented perpendicular to the original axis. The net rotation of the polarization axis thus depends on the degree of circular dichroism plus any actual optical rotation by the sample.