Enhanced production of mouse hybridomas to picomoles of antigen using EL-4 conditioned media with an in vitro immunization protocol

Author:

Ma Michael,Wu Shuenn-Jue,Howard Maureen,Borkovec Alexej B.

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Biotechnology,Plant Science,Biotechnology,Cell Biology,Clinical Biochemistry,Developmental Biology

Reference13 articles.

1. Kohler, G.; Milstein, C. Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 256: 495–497; 1975.

2. Luben, R. A.; Brazeau, P.; Bohlen, P.; Guillemin, R. Monoclonal antibodies to hypothalamic growth hormone releasing factor with picomoles of antigen. Science 218: 887–889; 1982.

3. Luben, R. A.; Mohler, M. A. In vitro immunization as an adjunct to the production of hybridomas producing antibodies against the lymphokine osteoclast activating factor. J. of Immunology 17: 635–639; 1980.

4. Farrar, J. J.; Fuller-Farrar, J.; Simon, P. L.; Hilfiker, M. L.; Stadler, B. M.; Farrar, W. L. Thymoma production of T cell growth factor (interleukin 2). J. Immunology 125: 2555–2558; 1980.

5. Howard, M.; Farrar, J.; Hilfiker, M.; Johnson, B.; Takatsu, K.; Hamaoka, T.; Paul, W. E. Identification of a T cell-derived B cell growth factor distinct from interleukin 2. J. Exp. Med. 155: 914–923; 1982.

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