Digital PCR (dPCR) and qPCR mediated determination of transgene copy number in the forage legume white clover (Trifolium repens)
Author:
Funder
Dairy Australia
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,General Medicine
Link
https://link.springer.com/content/pdf/10.1007/s11033-021-06354-5.pdf
Reference33 articles.
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2. Spangenberg G, Kalla R, Lidgett A, Sawbridge T, Ong EK, John U (2001) Transgenesis and genomics in molecular breeding of forage plants. Molecular breeding of forage crops. Kluwer Academic Publishers, Dordrecht, pp 1–28
3. Woodfield DR, White DWR (1996) Breeding strategies for developing transgenic white clover cultivars. Agronomy Society of New Zealand Special Publication 11/Grassland Research and Practice Series No. 6(11):125–130
4. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98(3):503–517. https://doi.org/10.1016/S0022-2836(75)80083-0
5. Głowacka K, Kromdijk J, Leonelli L, Niyogi KK, Clemente TE, Long SP (2016) An evaluation of new and established methods to determine T-DNA copy number and homozygosity in transgenic plants. Plant Cell Environ 39(4):908–917. https://doi.org/10.1111/pce.12693
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