Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors
Author:
Funder
Tehran University of Medical Sciences and Health Services
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,General Medicine
Link
https://link.springer.com/content/pdf/10.1007/s11033-023-08529-8.pdf
Reference27 articles.
1. Walsh G, Walsh E (2022) Biopharmaceutical benchmarks. Nat Biotechnol 40:1722–1760
2. Tihanyi B, Nyitray L (2021) Recent advances in CHO cell line development for recombinant protein production. Drug Discov Today Technol. https://doi.org/10.1016/j.ddtec.2021.02.003
3. Shin SW, Lee JS (2020) CHO cell line development and engineering via site-specific integration: challenges and opportunities. Biotechnol Bioprocess Eng. https://doi.org/10.1007/s12257-020-0093-7
4. Lee JS, Kildegaard HF, Lewis NE, Lee GM (2019) Mitigating clonal variation in recombinant mammalian cell lines. Trends Biotechnol 37(9):931–942
5. Banan M (2020) Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells. J Biotechnol 308:1–9
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