Another formula for calculating the gene change rate in real-time RT-PCR
Author:
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,General Medicine
Link
http://link.springer.com/content/pdf/10.1007/s11033-008-9430-1.pdf
Reference14 articles.
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2. Winer J, Jung CK, Shackel I et al (1999) Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem 270:41–49. doi: 10.1006/abio.1999.4085
3. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−DeltaDelta C(T)) method. Methods 25:402–408. doi: 10.1006/meth.2001.1262
4. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2002–2007. doi: 10.1093/nar/29.9.e45
5. Schefe JH, Lehmann KE, Buschmann IR et al (2006) Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula. J Mol Med 84:901–910. doi: 10.1007/s00109-006-0097-6
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