Expression of actively soluble antigen-binding fragment (Fab) antibody and GFP fused Fab in the cytoplasm of the engineered Escherichia coli-Reference-Cited by-同舟云学术

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Expression of actively soluble antigen-binding fragment (Fab) antibody and GFP fused Fab in the cytoplasm of the engineered Escherichia coli

Published:2020-05-11 Issue:6 Volume:47 Page:4519-4529
ISSN:0301-4851
Container-title:Molecular Biology Reports
language:en
Short-container-title:Mol Biol Rep

Author:

Krittanai Supaluk,Putalun Waraporn^ORCID,Sakamoto Seiichi^ORCID,Tanaka Hiroyuki,Juengwatanatrakul Thaweesak,Yusakul Gorawit^ORCID

Funder

The new strategic research (P2P) project, Walailak University

Faculty of Pharmaceutical Sciences, Graduate School of Khon Kaen University

National Research Council of Thailand

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,General Medicine

Link

https://link.springer.com/content/pdf/10.1007/s11033-020-05502-7.pdf

Reference35 articles.

1. Frenzel A, Hust M, Schirrmann T (2013) Expression of recombinant antibodies. Front Immunol 4:217. https://doi.org/10.3389/fimmu.2013.00217

2. Sockolosky JT, Szoka FC (2013) Periplasmic production via the pET expression system of soluble, bioactive human growth hormone. Protein Expres Purif 87(2):129–135. https://doi.org/10.1016/j.pep.2012.11.002

3. Robinson MP, Ke N, Lobstein J, Peterson C, Szkodny A, Mansell TJ, Tuckey C, Riggs PD, Colussi PA, Noren CJ, Taron CH, DeLisa MP, Berkmen M (2015) Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6(1):8072. https://doi.org/10.1038/ncomms9072

4. Lobstein J, Emrich CA, Jeans C, Faulkner M, Riggs P, Berkmen M (2012) SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microb Cell Fact 11:56. https://doi.org/10.1186/1475-2859-11-56

5. Yusakul G, Kitisripanya T, Juengwatanatrakul T, Sakamoto S, Tanaka H, Putalun W (2018) Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy. J Nat Med 72(3):641–650. https://doi.org/10.1007/s11418-018-1194-x

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1. Recent advancements in soluble expression of recombinant antibody fragments in microbial host systems;Preparative Biochemistry & Biotechnology;2024-08-28

2. Immunoaffinity separation of miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham using fragment antigen‐binding antibody produced via Escherichia coli;Phytochemical Analysis;2023-05-30

3. Comparative stability and analytical performance of anti-miroestrol recombinant antibody in different cassettes;Applied Microbiology and Biotechnology;2023-03-30

4. Improvement in the binding specificity of anti-isomiroestrol antibodies by expression as fragments under oxidizing conditions inside the SHuffle T7 E. coli cytoplasm;Bioscience, Biotechnology, and Biochemistry;2022-07-25

5. Lateral flow immunoassay for small-molecules detection in phytoproducts: a review;Journal of Natural Medicines;2022-02-16

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