Abstract
Abstract
Background
Multiple myeloma (MM) is a malignant proliferative disease of plasma cells, the incidence of which is increasing every year and remains incurable. The enzyme co-activator-associated arginine methyltransferase 1 (CARM1) is highly expressed in a variety of cancers, such as Hodgkin's lymphoma and acute myeloid leukemia, and CARM1 is closely associated with tumor cell proliferation. However, the role of CARM1 in MM has not been elucidated.
Methods and results
In this study, we found that CARM1 is overexpressed in MM and closely associated with poor prognosis in MM. CCK-8 and colony formation assays showed that the proliferation of MM cell lines was downregulated when CARM1 expression was knockdown by specific shRNA. Knockdown of CARM1 reduced the proportion of MM cell lines in the S phase and increased the proportion in G0/G1 phase. RNA-seq analysis of the CARM1-KD cell line revealed that it was closely associated with apoptosis and activated the p53 pathway. CCK-8 and apoptosis results showed that CARM1 knockdown made MM cells more sensitive to standard-of-care drugs.
Conclusion
This study provides an experimental basis for elucidating the pathogenesis of multiple myeloma and searching for potential therapeutic targets.
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,General Medicine
Reference35 articles.
1. Silberstein J, Tuchman S, Grant SJ (2022) What Is Multiple Myeloma?[J]. JAMA 327(5):497
2. Harding T, Baughn L, Kumar S et al (2019) The future of myeloma precision medicine: integrating the compendium of known drug resistance mechanisms with emerging tumor profiling technologies[J]. Leukemia 33(4):863–883
3. Oliva S, D’Agostino M, Boccadoro M et al (2020) Clinical Applications and Future Directions of Minimal Residual Disease Testing in Multiple Myeloma[J]. Front Oncol 10:1
4. Bonello F, Mina R, Boccadoro M et al (2019) Therapeutic Monoclonal Antibodies and Antibody Products: Current Practices and Development in Multiple Myeloma[J]. Cancers (Basel) 12:1
5. Dagostino M, Bertamini L, Oliva S et al (2019) Pursuing a Curative Approach in Multiple Myeloma: A Review of New Therapeutic Strategies[J]. Cancers (Basel) 11:12
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