A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR

Author:

Abdel Sater Fadi,Younes Mahmoud,Nassar Hassan,Nguewa Paul,Hamze KassemORCID

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,General Medicine

Reference11 articles.

1. Lopez-Rincon A, Alberto T, Lucero MM, Eric C et al (2020) Design of specific primer set for detection of B. 1.1. 7 SARS-CoV-2 variant using deep learning. bioRxiv. https://doi.org/10.1101/2020.12.29.424715

2. Erik V, Swapnil M, Meera C, Jeffrey CB, Robert J, Lily G, et al (2020) Transmission of SARS-CoV-2 lineage B. 1.1. 7 in England: insights from linking epidemiological and genetic data. medRxiv. https://doi.org/10.1101/2020.12.30.20249034

3. Meera C, Susan H, Gavin D, Hester A, Theresa L, Obaghe E et al (2020) Investigation of novel SARS-COV-2 variant: variant of concern 202012/01. Public Health England. https://www.gov.uk/government/publications/investigation-of-novel-sars-cov-2-variant-variant-of-concern-20201201

4. Andrew R, Nick L, Oliver P, Wendy B, Jeff B, Alesandro C et al (2020) Preliminary genomic characterisation of an emergent SARS-CoV-2 lineage in the UK defined by a novel set of spike mutations: COVID-19 genomics UK Consortium. https://virological.org/t/preliminary-genomic-characterisation-of-an-emergent-sars-cov-2-lineage-in-the-ukdefined-by-a-novel-set-of-spike-mutations/563

5. Afzal A (2020) Molecular diagnostic technologies for COVID-19: limitations and challenges. J Adv Res 26:149–159

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