Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2-Reference-Cited by-同舟云学术

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Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2

Published:2018-07-21 Issue:5 Volume:45 Page:1551-1556
ISSN:0301-4851
Container-title:Molecular Biology Reports
language:en
Short-container-title:Mol Biol Rep

Author:

Ramos-Benítez Marcos J.,Lopez-Cruz Luis M.,Aguayo Vasti,Ruiz-Jimenez Caleb,Espino Ana M.^ORCID

Funder

National Institute of Minority Health and Health Disparities

National Center on Minority Health and Health Disparities

National Sciences Fundation

Support of Competitive Research Program

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,General Medicine

Link

http://link.springer.com/article/10.1007/s11033-018-4251-3/fulltext.html

Reference24 articles.

1. Rivera F, Espino AM (2016) Adjuvant-enhanced antibody and cellular responses to inclusion bodies expressing FhSAP2 correlates with protection of mice to Fasciola hepatica. Exp Parasitol 160:31–38

2. Swartz J (2006) Developing cell-free biology for industrial applications. J Ind Microbiol Biotechnol 33:476–485

3. Caban-Hernandez K, Gaudier JF, Ruiz-Jimenez C, Espino AM (2014) Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis. J Clin Microbiol 52:766–772

4. Shin SH, Hsu A, Chastain HM, Cruz LA, Elder ES, Sapp SG, McAuliffe I, Espino AM, Handali S (2016) Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of human chronic fascioliasis. Am J Trop Med Hyg 95:852–855

5. Spirin AS (2004) High-throughput cell-free systems for synthesis of functionally active proteins. Trends Biotechnol 22:538–545

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