Abstract
AbstractActinidia seed-borne latent virus (ASbLV, Betaflexiviridae, genus Prunevirus) was detected at high frequency in healthy seedlings grown from lines of imported seed in a New Zealand post-entry quarantine facility. To determine the route and efficiency of transmission of ASbLV in this dioecious crop species, we developed a rapid molecular protocol and identified a reliable progeny plant tissue to determine paternal and maternal transmission rates. The virus was detected at a high incidence (98%) in individual seeds, but cotyledon testing of seedlings from selected crosses confirmed staminate (male) transmission at high frequency (~ 60%), and pistillate (female) transmission at even higher frequency (~ 80%). The use of cotyledons allows non-destructive detection of ASbLV in very young seedlings that enables early screening of kiwifruit plants in nurseries to manage its spread to orchards. The high ASbLV transmission rates, whether from infected pollen or ovules, facilitate bulk testing of seed lots that could quickly detect infected parent plants (fruit bearing female or male pollinator) already in an orchard. The dioecious nature of Actinidia may provide a useful biological tool to further investigate ASbLV movement, transmission biology, and ultimately its impact on infected Actinidia plants.
Funder
The New Zealand Institute for Plant And Food Research Limited
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Vine and Fruit Diseases;Kiwifruit;2023-11-30