The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time
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Published:2023-10-31
Issue:11
Volume:195
Page:
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ISSN:0167-6369
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Container-title:Environmental Monitoring and Assessment
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language:en
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Short-container-title:Environ Monit Assess
Author:
Rodrigues Elsa T.ORCID, Pereira EduardaORCID, Oliveira Paulo J.ORCID, Pardal Miguel A.ORCID
Abstract
AbstractThe present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To correlate standard analytical method results with in vitro results, a total of 16 physicochemical parameters, such as nutrients, pH, chemical oxygen demand, total suspended solids and metals, were determined in both raw and treated wastewater samples. Results revealed that the H9c2(2-1) cell-based SRB assay has an enormous potential to evaluate municipal wastewater quality over time and to discriminate influent and effluent toxic characteristics, as well as for water quality monitoring and surveillance of the efficacy of treatment processes. Finally, the gathered results alerted to the impact of phosphates in a biological system, leading us to recommend the selection of this parameter as a potential environmental health indicator.
Funder
Universidade de Coimbra
Publisher
Springer Science and Business Media LLC
Subject
Management, Monitoring, Policy and Law,Pollution,General Environmental Science,General Medicine
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