Abstract
AbstractThis experiment aimed to assess the regulatory effects of treatment with Balanites aegyptiaca fruit ethanol extract (BA-EE) on oxidant/antioxidant status, anti-inflammatory cytokines, and cell apoptosis gene expression in the abomasum of Haemonchus contortus–infected goats. Twenty goat kids were assigned randomly to four equal groups: (G1) infected-untreated, (G2) uninfected-BA-EE-treated, (G3) infected-albendazole-treated, (G4) infected-BA-EE-treated. Each goat in (G1), (G3), and (G4) was orally infected with 10,000 infective third-stage larvae. In the fifth week postinfection, single doses of albendazole (5 mg/kg.BW) and BA-EE (9 g/kg.BW) were given orally. In the ninth week postinfection, the animals were slaughtered to obtain abomasum specimens. The following oxidant/antioxidant markers were determined: malondialdehyde (MDA), glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT). The mRNA gene expression of cytokines (IL-3, IL-6, IL-10, TNF-α) and cell apoptosis markers (Bax, Bcl-2) were estimated. (G1) showed significantly reduced GSH content and GST and SOD activities but a markedly increased MDA level. (G3) and (G4) revealed a markedly lower MDA level with pronouncedly elevated GSH, SOD, and GST levels. The antioxidant properties of BA-EE were superior to those of albendazole. The mRNA gene expressions of IL-3, IL-6, IL-10, TNF-α, and Bax-2 were upregulated in (G1) but downregulated in (G3) and (G4). Bcl-2 and Bcl-2/Bax ratio expression followed a reverse course in the infected and both treated groups. We conclude that BA-EE treatment has a protective role in the abomasum of H. contortus–infected goats. This could be attributed to its antioxidant properties and ability to reduce pro-inflammatory cytokines and cell apoptosis.
Funder
National Research Centre Egypt
Publisher
Springer Science and Business Media LLC
Reference88 articles.
1. Abd El-Aziz TH, El-Beih NM, Soufy H et al (2014) Effect of Egyptian propolis on lipid profile and oxidative status in comparison with nitazoxanide in immunosuppressed rats infected with Cryptosporidium spp. Global Veterinaria 13:17–27
2. Abdel-Latif M, El‐Shahawi G, Aboelhadid SM et al (2017) Immunoprotective effect of chitosan particles on Hymenolepis nana–infected mice. Scand J Immunol 86:83–90
3. Abdel-Tawab H, Abdel-Haleem HM, Abdel-Baki AAS et al (2020) Anticoccidial and antioxidant activities of Moringa oleifera leaf extract on murine intestinal eimeriosis. Acta Parasitol 65:823–830
4. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
5. Ahmed WM, Mostafa AM, Al-Salahy MB et al (2019) The potential effect of Allium sativum and Coriandrum Sativum as natural sources of antioxidants on Haemonchus contortus infection in sheep. Egypt Veter Med Soc Parasitol J 15:89–113