An In Vitro Single-Primer Site-Directed Mutagenesis Method for Use in Biotechnology

Author:

Huang Yanchao,Zhang Likui

Publisher

Springer New York

Reference11 articles.

1. Hutchison CA 3rd, Phillips S, Edgell MH, Gillam S, Jahnke P, Smith M (1978) Mutagenesis at a specific position in a DNA sequence. J Biol Chem 253:6551–6560

2. Kunkel TA (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A 82:488–492

3. Kunkel TA, Roberts JD, Zakour RA (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154:367–382

4. Xia Y, Chu W, Qi Q, Xun L (2014) New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis. Nucleic Acids Res 43, e12

5. QuikChange® Multi Site-Directed Mutagenesis Kit. Instruction manual. http://www.genomics.agilent.com/files/Manual/200514.pdf

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