Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting

Author:

Alba F. Javier,Bartolomé Salvador,Bermúdez Antonio,Daban Joan-Ramon

Publisher

Springer New York

Reference15 articles.

1. Patton WF (2001) Detecting proteins in polyacrylamide gels and on electroblot membranes. In: Pennington SR, Dunn MJ (eds) Proteomics: from protein sequence to function. BIOS Scientific Publishers, Oxford, UK, pp 65–86

2. Daban J-R, Bartolomé S, Samsó M (1991) Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 199:169–174

3. Alba FJ, Bermúdez A, Bartolomé S, Daban J-R (1996) Detection of five nanograms of protein by two-minute Nile red staining of unfixed SDS gels. Biotechniques 21:625–626

4. Daban J-R, Bartolomé S, Bermúdez A, Alba FJ (2009) Rapid and sensitive staining of unfixed proteins in polyacrylamide gels with Nile red. In: Walker JM (ed) The protein protocols handbook, Third Edition pp 497–504

5. Greenspan P, Mayer EP, Fowler SD (1985) Nile red: a selective stain for intracellular lipid droplets. J Cell Biol 100:965–973

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