A method to select cell populations with enhanced chemotatic activity-Reference-Cited by-同舟云学术

A method to select cell populations with enhanced chemotatic activity

Published:1984-04-01 Issue:4 Volume:4 Page:311-318
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Albini Adriana¹,Müller Peter K.²,Parodi Silvio¹

Affiliation:

1. Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy

2. Max-Planck-Institut für Biochemie, 8033 Martinsried, West Germany

Abstract

Virus-transformed and tumor ceils often show a higher chemotactic activity in comparison to normal cells. Here we show that the blind-well chamber assay is an easy and rapid procedure to select cell populations with enhanced chemotactic activity. Collagen-type-specific antibodies have been applied to analyze the efficiency of cell sorting by the chemotaxis assay.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/4/4/311/471157/bsr0040311.pdf

Reference11 articles.

1. Albini A, Richter H & Pontz BF (1983) Localization of the chemotactic active domain in fibronectin. FEBS Lett.156, 22?226.

2. Alitalo K, Vaheri A, Krieg T & Timpl R (1980) Biosynthesis of two subunits of type IV procollagen and of other basement membrane proteins by a human tumor cell line. European Journal of Biochemistry109, 247?255.

3. Gay S, Martin GR, Müller PK, Timpl R, Kühn K (1976) Simultaneous synthesis of types I and III collagen by fibroblasts in culture. Proceedings of the National Academy of Sciences, U.S.A.73, 4037?4040.

4. Grinnel F, Billingham L & Burgess L (1981) Distribution of fibronectin during wound healing in vivo. Journal of Investigative Dermatology76, 181?189.

5. Ciba Foundation Symposium;NM Douarin Le,1976

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Modelling the collective response of heterogeneous cell populations to stationary gradients and chemical signal relay;Physical Biology;2017-11-16

2. The ‘chemoinvasion’ assay, 25 years and still going strong: the use of reconstituted basement membranes to study cell invasion and angiogenesis;Current Opinion in Cell Biology;2010-10

3. The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation;Nature Protocols;2007-03

4. Tumor and endothelial cell invasion of basement membranes;Pathology & Oncology Research;1998-09

5. Deficiencies in collagen phagocytosis by human fibroblasts in vitro: A mechanism for fibrosis?;Journal of Cellular Physiology;1993-06