Abstract
AbstractMany plants of commercial interest are propagated in tissue culture, with some presenting difficulties in their mineral balance. Historically, this issue has been addressed by varying the mineral composition of the media hoping to find a satisfactory combination. However, a potentially more efficient approach has been successful for several species, i.e., amendment of the media composition based on the mineral content of the plantlet leaves relative to that of the young leaves of thriving, field-grown plants. We used the latter approach to address a mineral imbalance encountered in propagating the Australian native plant, spotted iron gum (Corymbia maculata (syn. Eucalyptus maculata)). The chemical analysis showed that the concentration of iron in the plantlets was far in excess of requirement, and the plantlets thrived when its concentration in the medium was decreased 10-fold. This success supports the proposition that the mineral analysis of plantlets, together with the published data on foliar sufficiency ranges, may provide an efficient, general approach to optimizing the mineral composition of media for the in vitro production of plants.
Funder
Rural Industries Research and Development Corporation
Western Sydney University
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Biotechnology
Reference19 articles.
1. Ashrafi EN, Vahdati K, Ebrahaimzadeh H, Mirmasoumi M (2010) Analysis of in-vitro explants mineral contents to modify medium mineral composition for enhancing growth of Persian walnut (Juglans regia L.). J Food Agric Environ 8:325–329
2. Bryson GM, Mills HA (2014) Plant analysis handbook IV (e-Edition). A guide to sampling, preparation, analysis and interpretation for agronomic and horticultural crops. Macro-Micro Publishing, Inc., Athens, Georgia
3. Dell B, Robinson JM (1993) Symptoms of mineral nutrient deficiencies and nutrient concentrations in seedlings of Eucalyptus maculata Hook. Plant Soil 155:255–261
4. Gribble KD (1999) Understanding the physiological abnormality of tissue cultured plants known as vitrification. Ph.D. dissertation, University of Western Sydney, Penrith, New South Wales 1797, Australia
5. Gribble KD, Conroy JP, Holford P, Milham PJ (2002) In vitro uptake of minerals by Gypsophila paniculata and hybrid eucalypts and relevance to media mineral formulation. Aust J Bot 50:713–723