Rat bone marrow erythroid cell fractionation by counter current distribution in non-charge-sensitive two-phase systems-Reference-Cited by-同舟云学术

Rat bone marrow erythroid cell fractionation by counter current distribution in non-charge-sensitive two-phase systems

Published:1992-04-01 Issue:2 Volume:12 Page:77-85
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Garcia-Pérez A. I.¹,Sancho P.¹,Mendieta J.¹,Luque J.¹

Affiliation:

1. Departmento de Bioquímica y Biología Molecular, Universidad de Alcalá, Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain

Abstract

Counter-current distribution in non charge-sensitive aqueous poly(ethylene glycol)-dextran two phase systems allows the fractionation of rat bone marrow cells into two broad cell subpopulations with different distribution coefficients in a relatively short time. Morphological identification and enzymatic studies suggest that erythroid cells are mainly present in the subpopulation with the higher distribution coefficient. The distribution coefficient and, therefore, surface hydrophobicity of these cells, apparently increase in parallel with an increase in their degree of differentiation and maturation.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/12/2/77/468634/bsr0120077.pdf

Reference20 articles.

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3. Fisher, D. (1981) Biochem. J.196:1–10.

4. Fisher, D. and Sutherland, A. (1989)Separation using aqueous two-phase systems (Fisher, D. and Sutherland, A., eds.) Plenum Press, New York.

5. Guerci, O., Hout-Marchand, F. and Schneider, O. (1981)Scand. J. Haematol. 26:378–384.

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