Human cytomegalovirus multiple-strain infections and viral population diversity in haematopoietic stem cell transplant recipients analysed by high-throughput sequencing
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Published:2021-10-06
Issue:5-6
Volume:210
Page:291-304
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ISSN:0300-8584
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Container-title:Medical Microbiology and Immunology
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language:en
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Short-container-title:Med Microbiol Immunol
Author:
Dhingra A., Götting J., Varanasi P. R., Steinbrueck L., Camiolo S., Zischke J., Heim A., Schulz T. F., Weissinger E. M.ORCID, Kay-Fedorov P. C., Davison A. J., Suárez N. M., Ganzenmueller T.ORCID
Abstract
AbstractHuman cytomegalovirus (HCMV) is an important opportunistic pathogen in allogeneic haematopoietic stem cell transplant (HSCT) recipients. High-throughput sequencing of target-enriched libraries was performed to characterise the diversity of HCMV strains present in this high-risk group. Forty-four HCMV-DNA-positive plasma specimens (median viral input load 321 IU per library) collected at defined time points from 23 HSCT recipients within 80 days of transplantation were sequenced. The genotype distribution for 12 hypervariable HCMV genes and the number of HCMV strains present (i.e. single- vs. multiple-strain infection) were determined for 29 samples from 16 recipients. Multiple-strain infection was observed in seven of these 16 recipients, and five of these seven recipients had the donor (D)/recipient (R) HCMV-serostatus combination D + R + . A very broad range of genotypes was detected, with an intrahost composition that was generally stable over time. Multiple-strain infection was not associated with particular virological or clinical features, such as altered levels or duration of antigenaemia, development of acute graft-versus-host disease or increased mortality. In conclusion, despite relatively low viral plasma loads, a high frequency of multiple-strain HCMV infection and a high strain complexity were demonstrated in systematically collected clinical samples from this cohort early after HSCT. However, robust evaluation of the pathogenic role of intrahost viral diversity and multiple-strain infection will require studies enrolling larger numbers of recipients.
Funder
deutsche forschungsgemeinschaft deutsches zentrum für infektionsforschung medical research council wellcome trust hbrs graduate school Universitätsklinikum Tübingen
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Immunology,General Medicine,Immunology and Allergy
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