Abstract
AbstractTopmouth gudgeon is a freshwater fish species native to East Asia. Nowadays, P. parva is spread throughout Europe which is of concern because besides being considered one of the worst aquatic Invasive Alien Species (IAS) in Europe it is also a known vector of Spherotecum destruens, the rosette-like parasite lethal to other fish species. The present study describes the development and validation of a new species-specific assay based on hydrolysis probe chemistry to detect P. parva environmental DNA (eDNA) in water samples collected in a northern region of Italy (Friuli Venezia Giulia). Water samples were collected from 55 sites in an area where partial information on the occurrence of the species is available. eDNA was isolated from all samples and the presence of P. parva eDNA was tested by means of qPCR (quantitative PCR) and microfluidic qdPCR (quantitative digital PCR) techniques. Field results for both qPCR and qdPCR were largely in agreement in terms of detection (presence/absence). Thus, we judged the presence/absence by combining the results from the two methods and found that nine sites showed “strong positive” signal of P. parva eDNA (at least 2 positive replicates), 3 showed “suspected” (only 1 positive replicate), and 42 showed “absent”. The current study shows the strong potential of the newly developed eDNA approach to be a valuable addition to the monitoring of the highly invasive topmouth gudgeon in freshwater ecosystems.
Funder
Servizio biodiversità della Direzione centrale Risorse agroalimentari, forestali e ittiche RA-FVG
Publisher
Springer Science and Business Media LLC
Subject
Ecology,Ecology, Evolution, Behavior and Systematics
Reference39 articles.
1. Allard L, Greouillet G, Khazraie K, Tudesque K, Vigouroux R, Brosse S (2014) Electrofishing efficiency in low conductivity neotropical streams: towards a non-destructive fish sampling method. Fish Man Ecol 21:234–243
2. Boothroyd M, Mandrak NE, Fox M, Wilson CC (2016) Environmental DNA (eDNA) detection and habitat occupancy of threatened spotted gar (Lepisosteus oculatus). Aquat Conserv 26:1107–1119. https://doi.org/10.1002/aqc.2617
3. Cowart DA, Breedveld KGH, Ellis MJ, Hull JM, Larson ER (2018) Environmental DNA (eDNA) applications for the conservation of imperiled crayfish (Decapoda: Astacidea) through monitoring of invasive species barriers and relocated populations. J Crustacean Biol. https://doi.org/10.1093/jcbiol/ruy007
4. Cucchi F, Giorgetti F, Gemiti F, Massari G, Oberti S (1998) Caratterizzazione geochimica delle falde acquifere della pianura friulana (Geochemical caractherisation of the Friulian Plain aquifers). Atti Acque Sotterranee: Risorsa Invisibile 44:61–71
5. Cucchi F, Franceschini G, Zini L (2008) Hydrogeochemical investigations and groundwater provinces of the Friuli Venezia Giulia Plain aquifers, northeastern Italy. Environ Geol 55:985–999. https://doi.org/10.1007/s00254-007-1048-4
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献