Abstract
AbstractOur concerns have been raised about whether prolonged exposure to ammunition-related chemicals could correlate with genomic instability predisposing to lung carcinogenesis. The group of professional soldiers engaged in routine ammunition analysis and its explosive properties testing. To assess the presence of an innate genetic profile, DNA isolated from swabs was analyzed with LungCarta and HS Lung Panels and MassARRAY Analyzer 4 mass spectrometry. The presence of new somatic single nucleotide polymorphisms (SNPs) was detected with liquid biopsy technique and circulating cell-free DNA (ccfDNA) isolation, which was further analyzed with UltraSeek Lung Panel. Both genetic profiles obtained for each individual were compared. Genetic analysis revealed that in professional soldiers with long-term exposure to ammunition-related toxic chemicals, new SNPs in genes related to lung carcinogenesis are present. The preliminary data indicate that using modern molecular techniques could be a valuable tool for monitoring the genome instability in context of occupational risk of harmful volatile toxic compounds and improving personnel safety. The analyzed group will be further enlarged, and follow-up studies will be continued.
Publisher
Springer Science and Business Media LLC
Subject
Genetics,General Medicine
Reference19 articles.
1. Ackerman S, Horton W (2018) Chapter 2.4 Effects of environmental factors on DNA: damage and mutations. Green Chemistry, Elsevier: London, United Kingdom pp.109–128. https://doi.org/10.2016/B978-0-12-809270-5.00005-4
2. Borander AK, Voie OA, Longva K, Danielsen TE, Grahnstedt S, Sandvik L, Kongerud J, Sikkeland LIB (2017) Military small arms fire in association with acute decrements in lung function. Occup Environ Med 74:639–644
3. Bronkhorst AJ, Ungerer V, Holdenrieder S (2019) The emerging role of cell-free DNA as a molecular marker for cancer management. Biomol Detect Quantif 17:100087. https://doi.org/10.1016/j.bdq.2019.100087
4. Campbell JA, Van Stipdonk M, Wetzel S, Kohlhepp D (2018) Analysis of metallic components of GSR from various types of ammunition and firearms utilizing an SEM-EDX. Proceedings of the 5th Annual Graduate Student Research Symposium, Duquesne University. https://dsc.duq.edu/gsrs/2018/proceedings/3. Accessed 03/12/2021
5. Drzyzga O (2004) Diphenylamine and derivatives in the environment: a review. Chemosphere 53(8):809–818. https://doi.org/10.1016/S0045-6535(03)00613-1