Circulating miR-26b-5p and miR-451a as diagnostic biomarkers in medullary thyroid carcinoma patients
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Published:2023-06-07
Issue:12
Volume:46
Page:2583-2599
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ISSN:1720-8386
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Container-title:Journal of Endocrinological Investigation
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language:en
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Short-container-title:J Endocrinol Invest
Author:
Besharat Z. M., Trocchianesi S., Verrienti A., Ciampi R., Cantara S., Romei C., Sabato C., Noviello T. M. R., Po A., Citarella A., Caruso F. P., Panariello I., Gianno F., Carpino G., Gaudio E., Chiacchiarini M., Masuelli L., Sponziello M., Pecce V., Ramone T., Maino F., Dotta F., Ceccarelli M., Pezzullo L., Durante C.ORCID, Castagna M. G., Elisei R., Ferretti E.ORCID
Abstract
Abstract
Purpose/methods
The determination of tumour biomarkers is paramount to advancing personalized medicine, more so in rare tumours like medullary thyroid carcinoma (MTC), whose diagnosis is still challenging. The aim of this study was to identify non-invasive circulating biomarkers in MTC. To achieve this goal, paired MTC tissue and plasma extracellular vesicle samples were collected from multiple centres and microRNA (miRNA) expression levels were evaluated.
Results
The samples from a discovery cohort of 23 MTC patients were analysed using miRNA arrays. Lasso logistic regression analysis resulted in the identification of a set of circulating miRNAs as diagnostic biomarkers. Among them, miR-26b-5p and miR-451a, were highly expressed and their expression decreased during follow-up in disease-free patients in the discovery cohort. Circulating miR-26b-5p and miR-451a were validated using droplet digital PCR in a second independent cohort of 12 MTC patients.
Conclusion
This study allowed the identification and validation of a signature of two circulating miRNAs, miR-26b-5p and miR-451a, in two independent cohorts reporting a significant diagnostic performance for MTC. The results of this study offer advancements in molecular diagnosis of MTC proposing a novel non-invasive tool to use in precision medicine.
Funder
Agenzia Italiana del Farmaco, Ministero della Salute Sapienza Università di Roma Università degli Studi di Roma La Sapienza
Publisher
Springer Science and Business Media LLC
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism
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