Abstract
Abstract
Background
The gastric microbiome and inflammation play a key role in gastric cancer (GC) by regulating the immune response in a complex manner and by inflammatory events supporting carcinogenesis. Meprin β is a zinc endopeptidase and participates in tissue homeostasis, intestinal barrier function and immunological processes. It influences local inflammatory processes, dysbiosis and the microbiome. Here, we tested the hypothesis that meprin β is expressed in GC and of tumor biological significance.
Patients and methods
Four hundred forty whole mount tissue sections of patients with therapy-naive GC were stained with an anti-meprin β antibody. The histoscore and staining pattern were analyzed for each case. Following dichotomization at the median histoscore into a “low” and “high” group, the expression was correlated with numerous clinicopathological patient characteristics.
Results
Meprin β was found intracellularly and at the cell membrane of GC. Cytoplasmic expression correlated with the phenotype according to Lauren, microsatellite instability and PD-L1 status. Membranous expression correlated with intestinal phenotype, mucin-1-, E-cadherin-, β-catenin status, mucin typus, microsatellite instability, KRAS mutation and PD-L1-positivity. Patients with cytoplasmic expression of meprin β showed a better overall and tumor-specific survival.
Conclusions
Meprin β is differentially expressed in GC and has potential tumor biological relevance. It might function as a tumor suppressor or promotor depending on histoanatomical site and context.
Funder
Universitätsklinikum Schleswig-Holstein - Campus Kiel
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Gastroenterology,Oncology,General Medicine
Reference41 articles.
1. Camargo MC, Figueiredo C, Machado JC. Review: gastric malignancies: basic aspects. Helicobacter. 2019;24(Suppl 1):e12642.
2. Robert-Koch-Institut. Zentrum für Krebsregisterdaten—Magenkarzinom. https://www.krebsdaten.de/Krebs/EN/Content/Cancer_sites/Stomach_cancer/stomach_cancer_node.html. 2019.
3. Robertson, E.S., Microbiome and Cancer. Current Cancer Research, ed. W. El-Deiry. 2019, Gewerbestrasse 11, 6330 Cham, Switzerland. 401
4. Smyth, et al. Gastric cancer. Lancet. 2020;396(10251):635–48. https://doi.org/10.1016/S0140-6736(20)31288-5.
5. Ferreira RM, et al. Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota. Gut. 2018;67(2):226–36.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献