Listeria monocytogenes detection on food contact surfaces: suitability of different swab materials

Abstract

AbstractThe high binding capacity of Listeria monocytogenes to food contact surfaces increases the risk of cross-contamination in food. In addition to appropriate cleaning and disinfection procedures, a suitable sampling plan and technique for the earliest possible detection are necessary for prevention. This paper evaluates the sensitivity of 3 swab materials (cotton, viscose and nylon-flocked) for the qualitative and quantitative detection of L. monocytogenes on food contact surfaces (100 cm2). A L. monocytogenes cocktail of 3 serotypes (IIa, IIb and IVb) was applied to stainless steel, polyvinyl chloride, polytetrafluoroethylene and high-density polyethylene surfaces at a concentration of approx. 1.0 × 101-1.0 × 102 CFU/100 cm2 and approx. 4.0 × 104 CFU/100 cm2. The surfaces were sampled after 15 min of incubation by 3 different swabs using the double-swab technique, and then stored for 4 and 24 h until processing. The results of the qualitative and quantitative tests showed a few statistically significant differences in the detectability of L. monocytogenes by different swab materials, which implies that the detection rate of L. monocytogenes on a certain food contact surfaces can be increased by using the respective most suitable swab.