A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis

Author:

Dong Liming,Li Congcong,Xing Zhenjuan,He Yuxuan,Xia Wei,Liu Yijun,Long Likun,Li FeiwuORCID

Funder

National Science and Technology Innovation 2030 - Major Project

Publisher

Springer Science and Business Media LLC

Reference41 articles.

1. Bacalhau FB, Dourado PM, Horikoshi RJ, Carvalho RA, Semeão A, Martinelli S, Berger GU, Head GP, Salvadori JR, Bernardi O (2020) Performance of genetically modified soybean expressing the Cry1A.105, Cry2Ab2, and Cry1Ac proteins against key lepidopteran pests in Brazil. J Econ Entomol 113(6):2883–2889. https://doi.org/10.1093/jee/toaa236

2. European Commission Regulation (EU) 1829/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. 268:24–28. https://eur-lex.europa.eu/eli/reg/2003/1829/oj. Accessed 22 Sept 2003

3. European Union Reference Laboratory for GM Food and Feed (EURL GMFF) (2016) Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR; JRC102143. http://gmocrl.jrc.ec.europa.eu/StatusOfDossiers.aspx

4. Fu W, Wei S, Wang C, Du Z, Zhu P, Wu X, Wu G, Zhu S (2017) A temperature-tolerant multiplex elements and genes screening system for genetically modified organisms based on dual priming oligonucleotide primers and capillary electrophoresis. Food Chem 229:396–402. https://doi.org/10.1016/j.foodchem.2017.02.088

5. GM AgbioInvestor Monitor (2024) Global GM Crop Area 2023 Review. https://gm.agbioinvestor.com. Accessed 25 July 2024

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