Author:
Chen Nai-wen,Gao Jin-lai,Li Hai-long,Xu Hong,Wu Ling-feng,Meng Fan-guo,Chen Wei,Cao Yi-fang,Xie Wen-hua,Zhang Xiao-qin,Liu Shi-hui,Jin Jing,He Yi,Lv Jian-wei
Abstract
Abstract
Purpose
To evaluate the effects of manganese superoxide dismutase (Mn-SOD) from thermophilic bacterium HB27 (name as Tt-SOD) on chemical cystitis.
Methods
Control and experimental rats were infused by intravesical saline or hydrochloric acid (HCl) on the first day of the experiments. Saline, sodium hyaluronate (SH) or Tt-SOD were infused intravesically once a day for three consequent days. On the fifth day, the rats were weighted and sacrificed following a pain threshold test. The bladder was harvested for histological and biochemical analyses.
Results
Tt-SOD could reduce the bladder index, infiltration of inflammatory cells in tissues, serum inflammatory factors and SOD levels, mRNA expression of inflammatory factors in tissues, and increase perineal mechanical pain threshold and serum MDA and ROS levels in HCl-induced chemical cystitis. Furthermore, Tt-SOD alleviated inflammation and oxidative stress by the negative regulation of the NF-κB p65 and p38 MAPK signaling pathway.
Conclusions
Intravesical instillation of Tt-SOD provides protective effects against HCl-induced cystitis.
Funder
Medical Scientific Research Foundation of Zhejiang Province
Jiaxing Medical Key Subject Funding of Zhejiang Province
Jiaxing Key Laboratory of Precise Diagnosis and Treatment of Urological Tumor
Research Fund of clinical medicine discipline construction from Jiaxing University
Research Foundation of the Affiliated Hospital of Jiaxing University
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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