Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling
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Published:2023-11-09
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ISSN:1073-6085
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Container-title:Molecular Biotechnology
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language:en
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Short-container-title:Mol Biotechnol
Author:
Du Naiyi, Li Daojuan, Zhao Wei, Liu YakunORCID
Abstract
AbstractThe aberrant expression of Stratifin (SFN) is intricately associated with the initiation and progression of numerous tumors. This study aims to investigate whether SFN regulates the metastasis of cervical cancer cells through the LIMK2/Cofilin signaling pathway. In this study, we compared the expression of SFN in normal cervical tissues and cervical carcinoma tissues. We established SFN overexpression and SFN silencing cellular models to assess the invasive and migratory capabilities of cervical cancer cells using transwell and scratch assays. YO-PRO-1/PI and EdU staining were employed to evaluate apoptotic and proliferative capacities, while Actin-Tracker Green-488 was utilized to investigate cytoskeletal remodeling. The expression levels of SFN, LIMK2, p-LIMK2, Cofilin, and p-Cofilin were examined through Western blotting and immunofluorescence. Our findings revealed elevated expression of SFN in cervical squamous cell carcinoma tissues. SFN overexpression was observed to enhance invasion and migration of cervical cancer cells, induce cytoskeletal remodeling, facilitate cell proliferation, and suppress apoptosis. Furthermore, SFN overexpression upregulated the expression levels of LIMK2, p-LIMK2, Cofilin, and p-Cofilin. Conversely, silencing SFN exerted opposite effects. SFN plays an important role in the diagnosis of cervical cancer. SFN can regulate cervical cancer cell proliferation, apoptosis, cytoskeletal remodeling and metastasis through LIMK2/Cofilin signaling.
Funder
Hebei Province Medical Science Research Youth Science and Technology Project
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology
Reference29 articles.
1. Lin, S., Gao, K., Gu, S., You, L., Qian, S., Tang, M., Wang, J., Chen, K., & Jin, M. (2021). Worldwide trends in cervical cancer incidence and mortality, with predictions for the next 15 years. Cancer, 127, 4030–4039. 2. Sharifian, K., Shoja, Z., & Jalilvand, S. (2023). The interplay between human papillomavirus and vaginal microbiota in cervical cancer development. Virol J., 20, 73. 3. Van Heusden, G. P. (2005). 14-3-3 proteins: Regulators of numerous eukaryotic proteins. IUBMB Life, 57, 623–629. 4. Fan, X., Cui, L., Zeng, Y., Song, W., Gaur, U., & Yang, M. (2019). 14-3-3 proteins are on the crossroads of cancer, aging, and age-related neurodegenerative disease. International Journal of Molecular Sciences, 20, 3518. 5. Laronga, C., Yang, H. Y., Neal, C., & Lee, M. H. (2000). Association of the cyclin-dependent kinases and 14-3-3 sigma negatively regulates cell cycle progression. Journal of Biological Chemistry, 275, 23106–23112.
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