Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of Escherichia coli hosts during recombinant protein production

Author:

Nemecek Sabine,Marisch Karoline,Juric Renata,Bayer Karl

Publisher

Springer Science and Business Media LLC

Subject

General Medicine,Bioengineering,Biotechnology

Reference27 articles.

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2. Blattner FR, Pukett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997) The complete genome sequence of Escherichia coli K-12. Science 277(5331):1453–1474

3. Bentley WE, Mirjalili N, Andersen DC, Davis RH, Kompala DS (1990) Plasmid encoded protein: the principal factor in the “metabolic burden” associated with recombinant bacteria. Biotechnol Bioeng 35:668–681

4. Wick LM, Egli T (2004) Molecular components of physiological stress responses in Escherichia coli. Adv Biochem Eng Biochnol 89:1–45

5. Cashel M, Rudd KE (1987) The stringent response. In: Neidhardt FC, Ingraham JL, Broosk Low K, Magasanik B, Schächter M, Umbarger H (eds) Escherichia coli and Salmonella typhimurim: cellular and molecular biology. American Society of Microbiology, Washington DC pp 1410–1438

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