Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr-Reference-Cited by-同舟云学术

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Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr

Published:2021-08-09 Issue:9 Volume:11 Page:
ISSN:2190-572X
Container-title:3 Biotech
language:en
Short-container-title:3 Biotech

Author:

Wang Zhiqiang,Yu Fangqin,Shi Dingding,Wang Ying,Xu Feng,Zeng Shaohua^ORCID

Funder

Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases

Publisher

Springer Science and Business Media LLC

Subject

Agricultural and Biological Sciences (miscellaneous),Environmental Science (miscellaneous),Biotechnology

Link

https://link.springer.com/content/pdf/10.1007/s13205-021-02954-x.pdf

Reference41 articles.

1. Bansal R, Mittapelly P, Cassone BJ, Mamidala P, Redinbaugh MG, Michel A (2015) Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses. PLoS ONE 10(8):e0134890. https://doi.org/10.1371/journal.pone.0134890

2. Borges A, Tsai SM, Caldas DG (2012) Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Plant Cell Rep 31(5):827–838. https://doi.org/10.1007/s00299-011-1204-x

3. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55(4):611–622. https://doi.org/10.1373/clinchem.2008.112797

4. Cheng X-x, Tang X-m, Guo C-c, Zhang C-r, Yang Q (2018) New flavonol glycosides from the seeds of Desmodium styracifolium. Chem Nat Compd 54(5):846–850. https://doi.org/10.1007/s10600-018-2496-7

5. Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Can Res 64:5245–5250

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. RNA-seq validation: software for selection of reference and variable candidate genes for RT-qPCR;BMC Genomics;2024-07-16

2. Desmodium styracifolium: Botanical and ethnopharmacological insights, phytochemical investigations, and prospects in pharmacology and pharmacotherapy;Heliyon;2024-02

3. Understanding the role of GsWRKY transcription factors modulating the biosynthesis of schaftoside in Grona styracifolia;Horticulture Advances;2023-12-21

4. Identification of Reliable Reference Genes under Different Stresses and in Different Tissues of Toxicodendron succedaneum;Genes;2022-12-17

5. Systematic Identification and Validation of Suitable Reference Genes for the Normalization of Gene Expression in Prunella vulgaris under Different Organs and Spike Development Stages;Genes;2022-10-25

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