Abstract
AbstractThe current report assesses the efficiency of encapsulation–desiccation protocol to cryopreserve oil palm (Elaeis guineensis Jacq.) polyembryoids. Specifically identified polyembryoids, comprising of haustorium and torpedo-shaped structures, were encapsulated [comprising 3% (w/v) sodium alginate and 100 mM CaCl2]. Calcium alginate-encapsulated and sucrose-precultured polyembryoids were subjected to different spans of desiccation in a laminar air-flow cabinet, followed by freezing in liquid nitrogen. The effect of sucrose preculture (with gradual exposure to 0.3, 0.5, 0.75 and 1 M for 7 days) and dehydration periods (0–10 h) under sterile air-flow on post-freezing survival and regrowth of encapsulated polyembryoids were studied. Cryopreserved and thawed polyembryoids (initially precultured in sucrose, followed by 9 h air-desiccated to 23.3% moisture content) displayed the highest survival percentage (73.3%) and regeneration (of shoot, root and secondary somatic embryo) on Murashige and Skoog regrowth medium containing sucrose (0.3–1 M) and 0.2 mg/l 2,4-dichlorophenoxy acetic acid. In addition, ultrastructural study using scanning electron microscopy exhibited successful revival of cryopreserved polyembryoids, owing to retention of cellular membrane stability through optimized and protected (encapsulated) desiccation. The present study thus substantiates the potential of this encapsulation–desiccation procedure in cryopreservation of oil palm polyembryoids for long-term conservation programs.
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Environmental Science (miscellaneous),Biotechnology
Reference35 articles.
1. Barbara MR, Laura S, Dominique D, Erica EB (2005) Evaluation of a modified encapsulation-dehydration procedure incorporating sucrose pretreatments for the cryopreservation of Ribes germplasm. Vitro Cell Dev Biol Plant 41:431–436
2. Benson EE (2008) Cryopreservation theory. In: Reed BM (ed) Plant cryopreservation: a practical guide. Springer, New York, pp 15–32
3. Carpenter JF, Clegg JS, Crowe JH, Somero GN (1993) Compatible solutes and macromolecular stability. Cryobiology 30:201–241
4. Dereuddre J, Scottez C, Arnaud Y, Duron M (1990) Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv Beurré Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardiness. In: Comptes Rendus de l’Académie des Sciences Paris, t. 310, vol 3, pp 317–323
5. Dumet D, Engelmann F, Chabrilange N, Duval Y, Dereuddre J (1993a) Importance of sucrose for the acquisition of tolerance to desiccation and cryopreservation of oil palm somatic embryos. CryoLetters 14:243–250
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献