Transcriptomic approaches for identifying potential transmission blocking vaccine candidates in Plasmodium falciparum: a review of current knowledge and future directions
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Published:2023-09-12
Issue:10
Volume:13
Page:
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ISSN:2190-572X
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Container-title:3 Biotech
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language:en
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Short-container-title:3 Biotech
Author:
Varijakshi GutthedharORCID, Divya Mallya, Ware Akshay PramodORCID, Paul BobbyORCID, Saadi Abdul VahabORCID
Abstract
AbstractUtilizing transcriptomics, promising methods for identifying unique genes associated with Plasmodium gametocyte development offer a potential avenue for novel candidate targets in transmission blocking vaccine development. In this review, we identified 40 publicly available transcriptomic datasets related to parasite factors linked with sexual stage transmission, from which we analyzed two RNA-Seq datasets to identify potential genes crucial for the transmission of P. falciparum from humans to mosquito vectors. Differential expression analysis revealed 3500 (2489 upregulated and 1011 downregulated) common genes differentially expressed throughout sexual stage development of P. falciparum occurring in both humans (gametocyte stage II, V) and mosquitoes (ookinete). Among which 1283 (914 upregulated and 369 downregulated) and 826 (719 upregulated and 107 downregulated) genes were specific to female and male gametocytes, respectively. Also, 830 potential transition associated genes were identified that may be involved in the adaptation and survival of the parasite in between human and mosquito stages. Additionally, we reviewed the functional aspects of important genes highly expressed throughout the sexual stage pathway and evaluated their suitability as vaccine candidates. The review provides researchers with insight into the importance of publicly available transcriptomic datasets for identifying critical and novel gametocyte markers that may aid in the development of rational transmission blocking strategies.
Funder
Manipal Academy of Higher Education, Manipal
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Environmental Science (miscellaneous),Biotechnology
Reference43 articles.
1. Angrisano F, Sala KA, Da DF, Liu Y, Pei J, Grishin NV, Snell WJ, Blagborough AM (2017) Targeting the conserved fusion loop of HAP2 inhibits the transmission of Plasmodium berghei and falciparum. Cell Rep 21:2868–2878. https://doi.org/10.1016/j.celrep.2017.11.024 2. Barry A, Bradley J, Stone W, Guelbeogo MW, Lanke K, Ouedraogo A, Soulama I, Nébié I, Serme SS, Grignard L, Patterson C, Wu L, Briggs JJ, Janson O, Awandu SS, Ouedraogo M, Tarama CW, Kargougou D, Zongo S, Bousema T (2021) Higher gametocyte production and mosquito infectivity in chronic compared to incident Plasmodium falciparum infections. Nat Commun 12:2443. https://doi.org/10.1038/s41467-021-22573-7 3. Bennink S, von Bohl A, Ngwa CJ, Henschel L, Kuehn A, Pilch N, Weißbach T, Rosinski AN, Scheuermayer M, Repnik U, Przyborski JM, Minns AM, Orchard LM, Griffiths G, Lindner SE, Llinás M, Pradel G (2018) A seven-helix protein constitutes stress granules crucial for regulating translation during human-to-mosquito transmission of Plasmodium falciparum. PLoS Pathog 14:e1007249. https://doi.org/10.1371/journal.ppat.1007249 4. Bradley J, Stone W, Da DF, Morlais I, Dicko A, Cohuet A, Guelbeogo WM, Mahamar A, Nsango S, Soumaré HM, Diawara H, Lanke K, Graumans W, Siebelink-Stoter R, van de Vegte-Bolmer M, Chen I, Tiono A, Gonçalves BP, Gosling R, Bousema T (2018) Predicting the likelihood and intensity of mosquito infection from sex specific Plasmodium falciparum gametocyte density. Elife 7:e34463. https://doi.org/10.7554/eLife.34463 5. Chandley P, Ranjan R, Kumar S, Rohatgi S (2023) Host-parasite interactions during Plasmodium infection: implications for immunotherapies. Front Immunol 13:1091961. https://doi.org/10.3389/fimmu.2022.1091961
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