Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase-Reference-Cited by-同舟云学术

Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase

Published:1996-04-01 Issue:2 Volume:16 Page:107-113
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Reis Eduardo M. R.¹,Slayman Carolyn W.²,Verjovski-Almeida Sergio¹

Affiliation:

1. Departamento de Bioquímica, Instituto de Química, Universidade de Sāo Paulo, 05508-900 Sāo Paulo, S.P., Brazil.

2. Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.

Abstract

In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca2+-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/16/2/107/469217/bsr0160107.pdf

Reference20 articles.

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