Comparison of deconvoluted plasma DSC curves on patients with solid tumors

Abstract

AbstractToday, differential scanning calorimetry (DSC) has increasingly been an accepted method for diagnosing and monitoring different diseases. Moreover, as a highly sensitive technique it seems to be applicable in human cancer researches. Our workgroup in several previous researches has already demonstrated marked differences on DSC plasma profiles between healthy control and various tumorous patients and, in addition, at their different stages. The aim of this study was to compare deconvoluted plasma DSC curves on patients with solid tumors. Blood plasma DSC data were analyzed retrospectively from various patients, who has only local cancer diseases (malignant melanoma: n = 15, breast carcinoma: n = 10 and pancreas adenocarcinoma: n = 11) without any regional or distant metastases. The complex curves were deconvoluted in numerous individual transitions (five or seven melting points). In the examined patients with solid tumors, the thermodynamic parameters: heat flow and calorimetric enthalpy of the transitions corresponding to the most abundant plasma proteins, as well as the numbers of transitions were determined from the calorimetric profiles. Deconvoluted DSC curves from blood samples of patients showed differences and similarities at the same time in the thermal denaturation of plasma components in various carcinoma cases.