Different responses of lipids and lignin phenols to nitrogen addition in meadow grassland soil

Abstract

Abstract Purpose Nitrogen (N) enrichment can affect the composition and stability of soil organic carbon (SOC) pools by altering vegetation and soil properties. However, the response of plant-derived carbon components in soil to different N addition levels is unclear. We investigated the changes and potential driving processes of plant-derived carbon components (especially lignins and lipids) in meadow grassland soils under long-term N addition in eastern Inner Mongolia, China. Materials and methods Biomarker technology was utilised to analyse changes in plant-derived carbon components (C>20 free lipids, bound lipids, and lignin phenols) in soil under different N addition levels, including changes in soil chemical properties, enzyme activity, plant biomass, and diversity under N addition, as well as the specific pathways involved. Results and discussion We found that high levels of N addition significantly reduced the concentration of soil lignin phenols whereas increased the accumulation of lipids (free and bound lipids). Compared with changes in plant biomass and diversity, soil chemical properties and enzyme activity play a more significant role in regulating the accumulation and degradation of plant-derived carbon. Structural equation modelling (SEM) showed that decreases in lignin phenol concentration were related to specific biochemical decomposition processes (increased polyphenol oxidase activity and decreased C/N). The increase in lipids associated with the protective effects of minerals mediated by pH. Conclusions In general, plant-derived carbon components showed inconsistent responses to N addition, lignin phenol concentration decreased and lipid concentration increased, which was mainly related to the change of soil biochemical properties. Plant-derived carbon components only showed significant changes under high N addition levels. Furthermore, our research indicates that SOC sequestration and functioning are highly dependent on soil biochemical properties, which weakens the influence of changes in plant carbon input on soil carbon storage.