Abstract
AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high.
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Ecology, Evolution, Behavior and Systematics
Reference144 articles.
1. Ahirwar S, Soni H, Prajapati BP, Kango N (2017) Isolation and screening of thermophilic and thermotolerant fungi for production of hemicellulases from heated environments. Mycol 8:125–134. https://doi.org/10.1080/21501203.2017.1337657
2. Ali M, Moghazy S, Shaban G, El-Sababty Z (2009) Heat-resistant fungi isolated from soil in Minia Governorate. Assiut Univ J Bot 38:93–106
3. Anastasi A, Varese GC, Filipello Marchisio V (2005) Isolation and identification of fungal communities in compost and vermicompost. Mycologia 97:33–44. https://doi.org/10.1080/15572536.2006.118328362006.11832836
4. Apinis AE (1963) Thermophilous fungi of coastal grasslands. In: Doeksen J, Van der Drift J (eds) Soil Organisms, North-Holland Publishing Co, Amsterdam, pp 427–438
5. Balajee SA, Gribskov J, Brandt M, Ito J, Fothergill A, Marr KA (2005) Mistaken identity. Neosartorya pseudofischeri and its anamorph masquerading as Aspergillus fumigatus. J Clin Microbiol 43:5996–5999. https://doi.org/10.1128/JCM.43.12.5996-5999.2005
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