Abstract
AbstractFungal specimens parasitic on spider egg sacs (Araneidae sensu lato) were collected, isolated, and identified based on molecular phylogenetic analyses of five nuclear loci (ITS, LSU, TEF1, RPB1 and RPB2) combined with morphological data. In this study, one novel monotypic genus is described, Bhushaniella rubra for Thailand. Bhushaniella rubra is characterized by producing superficial perithecia. Its anamorph has a unique character by producing verticillate phialides with a slightly curved neck. A concurrent evaluation of the secondary metabolites of the mycelial extracts of the new fungus revealed the presence of picoline alkaloids of the penicolinate type, for which we propose the trivial names penicolinates F and G. Their chemical structures were elucidated by two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and high resolution mass spectrometry (HR-MS). They only showed weak to no antibiotic activity and were devoid of significant cytotoxic effects.
Funder
Horizon 2020 Framework Programme
National Science and Technology Development Agency
Alexander von Humboldt-Stiftung
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Ecology, Evolution, Behavior and Systematics