Two-dimensional structure of phospholipase induced crystals of Ca2+-ATPase from sarcoplasmic reticulum-Reference-Cited by-同舟云学术

Two-dimensional structure of phospholipase induced crystals of Ca2+-ATPase from sarcoplasmic reticulum

Published:1986-12-01 Issue:12 Volume:6 Page:1065-1070
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Misra M.¹,Malhotra S. K.¹

Affiliation:

1. Biosciences Electron Microscope Laboratory, Department of Zoology, University of Alberta, Edmonton, Alberta, Canada T6G 2E1

Abstract

A two-dimensional projection map was computed of the Ca2+-ATPase molecules in sarcoplasmic reticulum, isolated from rabbit skeletal muscle. Crystalline arrays of Ca2+-ATPase molecules were formed by incubating the membrane vesicles with phospholipase A2 and dialysing against Tris/HCl buffer. Ca2+-ATPase molecules appear as quasi-triangular blobs in the projection map and seem to form dimers. The projection map seems to indicate an enzyme conformation somewhat similar to vanadate-induced crystals but different from lanthanide-induced crystals of Ca2*-ATPase.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/6/12/1065/471503/bsr0061065.pdf

Reference13 articles.

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1. Chapter 3 The Ca2+ transport ATPases of sarco(endo)-plasmic reticulum and plasma membranes;Molecular Aspects of Transport Proteins;1992