Validation of the qPCR technique for the detection of Plasmodiophora brassicae in horticultural soils of Colombia

Author:

García-Arias Francy LilianaORCID,Rodríguez EdwinORCID,Dávila-Mora LorenaORCID,Riascos-Ortiz DonaldORCID,Revelo-Gómez ElianaORCID,Gelvez Alejandro VillabonaORCID,Moreno-Velandia Carlos AndrésORCID,Zuluaga PaolaORCID

Publisher

Springer Science and Business Media LLC

Reference40 articles.

1. Al-Daoud F, Gossen B, Robson J, McDonald M (2017) Propidium monoazide improves quantification of resting spores of Plasmodiophora brassicae with qPCR. Plant Disease 101:442–447

2. Alvarez G, Gonzalez M, Isbal S, Blanc V, Leon R (2013) Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide. AMB Express 3:1

3. Botero-Ramírez A, Padilla-Huertas FL, Strelkov SE, García-Dominguez C (2022) The Occurrence of Clubroot in Colombia and Its Relationship with Climate and Agronomic Practices. Horticulturae 8:1–12

4. Cao T, Tewari J, Strelkov SE, Science N, Tg AB (2007) Molecular Detection of Plasmodiophora brassicae, Causal Agent of Clubroot of Crucifers. Plant and Soil Plant Disease 91:5–12

5. Cubeta M, Porter D, Mozley-Standridge S (2008) Laboratory Exercises with Zoosporic Plant Pathogens. In: Trigiano R, Windham M, Windham A (eds) Plant pathology: concepts and laboratory exercises, 2nd ed CRC Press, p 558

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