Author:
Guan Xin,Lance Richard F.,Feist Sheena M.
Abstract
AbstractWe designed two novel environmental DNA (eDNA) assays for the detection of Ridgway’s Rail (Rallus obsoletus), and successfully validated each assay using eDNA samples collected from the species’ known distribution within the United States. These assays add to the suite of tools available for the monitoring of this rare and secretive marsh bird, and may help to further elucidate its movement patterns as well as identify important migration corridors. Observed sensitivity of the assays indicates exceptional performance, with limits of detection at ≤ 8 copies of the target eDNA fragment per reaction. Our publication adds to the growing body of literature supporting eDNA surveys as viable tools for bird monitoring endeavors.
Funder
Department of Defense, Strategic Environmental Research and Development Program
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Ecology, Evolution, Behavior and Systematics
Reference29 articles.
1. Beng KC, Corlett RT (2020) Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects. Biodivers Conserv 29:2089–2121. https://doi.org/10.1007/s10531-020-01980-0
2. Carim KJ, McKelvey KS, Young MK et al (2016) A protocol for collecting environmental DNA samples from streams. Gen. Tech. Rep. RMRS-GTR-355. US Department of Agriculture, Forest Service, Rocky Mountain Research Station, Fort Collins, CO
3. Casazza ML, Overton CT, Bui TD et al (2016) Depredation of the California Ridgeway’s rail: causes and distribution. Proc Vertebrate Pest Conf 27. https://doi.org/10.5070/V427110553
4. Chesser RT, Banks RC, Cicero C et al (2014) Fifty-fifth supplement to the American ornithologists’ Union check-list of North American Birds. Auk 131:CSi–CSxv. https://doi.org/10.1642/AUK-14-124.1
5. Conway CJ (2009) Standardized North American Marsh Bird Monitoring Protocols, v. 2009-2. Wildlife Research Report #2009-02. US Geological Survey, Arizona Cooperative Fish and Wildlife Research Unit, Tucson, AZ. https://cales.arizona.edu/research/azfwru/NationalMarshBird/index.htm
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献