Development of new SNPs loci on Quercus robur and Quercus petraea for genetic studies covering the whole species’ distribution range

Author:

Blanc-Jolivet Celine,Bakhtina Svetlana,Yanbaev Ruslan,Yanbaev Yulai,Mader Malte,Guichoux Erwan,Degen Bernd

Abstract

AbstractWe used double digest restriction site associated DNA sequencing (ddRAD) to develop new geographically informative nuclear SNP loci in Quercus robur and Quercus petraea. Genotypes derived from sequence data of 95 individuals covering the distribution range of the species were analysed to select geographically informative and polymorphic loci within Russia and Germany. We successfully screened a selected set of 119 loci on a MassARRAY® iPLEX™ platform on 190 individuals from 19 locations in Russia. The newly developed loci will be useful for genetic studies over the whole distribution range of both species.

Funder

Bundesanstalt für Landwirtschaft und Ernährung

Russian Science Foundation

ANR

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Ecology, Evolution, Behavior and Systematics

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