Holocrine secretion and kino flow in angiosperms: their role and physiological advantages in plant defence mechanisms

Abstract

Abstract Key message Flow within internal secretory systems derived from autolyzing epithelia in kino-producing angiosperms may have lower metabolic energy costs. This presents physiological advantages and possibly constitutes an evolutionary step of these species compared to resin flow in conifers in using internal secretory systems in plant defence mechanisms. Abstract Kino is a plant exudate, rich in polyphenols, produced by several angiosperms in reaction to injury of the cambium. It flows out of kino veins, which compose an anatomically distinct continuous system of tangentially anastomosing canals produced by the cambium upon damage, encircling plant stems and branches. Kino is loaded into the vein lumen by autolysis of a cambiform epithelium lined by suberized cells that separate kino veins from the surrounding axial parenchyma. A model describing kino flow is presented to investigate how vein distribution and structure, as well as the loading, solidification, and viscosity of kino affect flow. Considering vein anatomy, viscosity, and a time-dependent loading of kino, the unsteady Stokes equation was applied. Qualitatively, kino flow is similar to resin flow observed on conifers. There is an increase in flow towards the vein open end, with both pressure and flow depending on the vein dimensions, properties, and loading of kino. However, kino veins present a much smaller specific resistance to flow compared to resin ducts. Also, unlike resin loading in conifers, the loading of kino is not pressure-driven. The pressure and pressure gradient required to drive an equally fast flow are smaller than what is observed on the resin ducts of conifers. These results agree with previous observations on some angiosperms and suggest that flow within internal secretory systems derived from autolysing epithelia may have lower metabolic energy costs; thus presenting physiological advantages and possibly constituting an evolutionary step of angiosperms in using internal secretory systems in plant defence mechanisms compared to resin flow in conifers. Understanding of how these physiological and morphological parameters affect kino flow might be useful for selecting species and developing more sustainable and economically viable methods of tapping gum and gum resin in angiosperms.