The use of tetrazolium salt-based methods for determination of islet cell viability in response to cytokines: a cautionary note
Author:
Publisher
Springer Science and Business Media LLC
Subject
Endocrinology, Diabetes and Metabolism,Internal Medicine
Link
http://link.springer.com/content/pdf/10.1007/s00125-004-1566-5.pdf
Reference8 articles.
1. Janjic D, Wollheim CB (1992) Islet cell metabolism is reflected by the MTT (tetrazolium) colorimetric assay. Diabetologia 35:482?485
2. Janjic D, Asfari M (1992) Effects of cytokines on rat insulinoma INS-1 cells. J Endocrinol 132:67?76
3. Chen G, Hohmeier HE, Newgard CB (2001) Expression of the transcription factor STAT-1 alpha in insulinoma cells protects against cytotoxic effects of multiple cytokines. J Biol Chem 276:766?772
4. Lortz S, Tiedge M, Nachtwey T, Karlsen AE, Nerup J, Lenzen S (2000) Protection of insulin-producing RINm5F cells against cytokine-mediated toxicity through overexpression of antioxidant enzymes. Diabetes 49:1123?1130
5. Karlsen AE, Ronn SG, Lindberg K et al. (2001) Suppressor of cytokine signaling 3 (SOCS-3) protects beta-cells against interleukin-1beta- and interferon-gamma-mediated toxicity. Proc Natl Acad Sci USA 98:12191?12196
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