A perfusion protocol for highly efficient transduction of intact pancreatic islets of Langerhans
Author:
Publisher
Springer Science and Business Media LLC
Subject
Endocrinology, Diabetes and Metabolism,Internal Medicine
Link
http://link.springer.com/content/pdf/10.1007/s00125-006-0390-5.pdf
Reference9 articles.
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2. Jansson L (1986) Flow distribution between the endocrine and exocrine parts of the isolated rat pancreas during perifusion in vitro with different glucose concentrations. Acta Physiol Scand 126:533–538
3. He T, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B (1998) A simplified system for generating recombinant adenoviruses. Proc Natl Acad Sci USA 95:2509–2514
4. Mostoslavsky G, Kotton DN, Fabian AJ, Gray JT, Lee JS, Mulligan RC (2005) Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulation. Mol Ther 1:932–940
5. Barbu AR, Akusjarvi G, Welsh N (2005) Adenoviral-mediated transduction of human pancreatic islets: importance of adenoviral genome for cell viability and association with a deficient antiviral response. Endocrinology 146:2406–2414
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