Author:
Bury Alexander G.,Pyle Angela,Marcuccio Fabio,Turnbull Doug M.,Vincent Amy E.,Hudson Gavin,Actis Paolo
Abstract
Abstract
Intracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease, it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here, we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state-of-the-art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of >20 µm for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.
Graphical abstract
Funder
Wellcome Trust
H2020 Marie Skłodowska-Curie Actions
Medical Research Council
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Analytical Chemistry
Cited by
5 articles.
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