Simple workflow to repurpose SARS-CoV-2 swab/serum samples for the isolation of cost-effective antibody/antigens for proteotyping applications and diagnosis
Author:
Funder
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu
Kalkinma Bakanliği
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Analytical Chemistry
Link
https://link.springer.com/content/pdf/10.1007/s00216-021-03654-4.pdf
Reference27 articles.
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2. Read AJ, Gauci CG, Lightowlers MW. Purification of polyclonal anti-conformational antibodies for use in affinity selection from random peptide phage display libraries: a study using the hydatid vaccine EG95. J Chromatogr B Anal Technol Biomed Life Sci. 2009;877(14–15):1516–22.
3. Kuravsky ML, Schmalhausen EV, Pozdnyakova NV, Muronetz VI. Isolation of antibodies against different protein conformations using immunoaffinity chromatography. Anal Biochem. 2012;426(1):47–53.
4. Chen RE, Zhang X, Case JB, Winkler ES, Liu Y, VanBlargan LA, et al. Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonalantibodies. Nat Med 27 (4):717–26.https://doi.org/10.1038/s41591-021-01294-w.
5. McMahon DE, Peters GA, Ivers LC, Freeman EE. Global resource shortages during COVID-19: bad news for low-income countries. PLoS Negl Trop Dis. 2020;14(7):e0008412.
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